Flow Cytometry Introduction - Malte Paulsen (EMBL)

Thank you for the great explaination!

one size FITC all

Dang! That was fascinating and scarily understandable, thank you!!!

Mn wojoohahom ta3refoonahom. Even their dialogue.. No need for cytometry

Thank you iBiology and Dr. Paulsen for this very useful and information-rich video!

I watched this when I was learning flow and continue to send it to other grad students. Such a great video!

Watched a lot of videos before coming across yours, and now i can finally sat I understand how it works. Thank you keep making great content like this.

Very nice video! Thanks!

Excellent video. I wish the one was released in 2010 when I started my PhD. Had I seen this one, I would had not made so many avoidable mistakes.

Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer?
This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)

Personally speaking it's the best flow cytometry introductory video so far. The mechanisms are explained in a clear yet informative way. Details like channel resolution, flow structure etc are included which is scarce in other similar videos. Thanks!

Amazing

it is very informative and simpified. many thanks malte

Really nice and clear explanation. Congratulation. Looking forward for a video into multiparametric analysis of data

SO nice and helpful

awesome mate. Thanks

GREAT!

well done!

Great information and video

Super informative video! Just wondering but isn't it 45 bivariate combinations with 10 antibodies?

Thanks Sir you and your video is very nice. Your explanation method is best. Sir please explain between PE-CD4 and FITC-CD3 .

Awesome video! Subtitle required. I can't find Auto-subtitle option.

Awesome vedieo

Excellent video

great video

A question, In the Hydrodynamic focusing, Is the sheath fluid merging with the blood sample (which already diluted) if yes, then the dilution ratio will be changed? and if no, How they are not merging ! TIA

Thank you

thank you, this was very helpful

I loved this video, it's very nicely explained and the animations are great. Just one minor comment that 2^4 goes to 16 channels. Thanks for sharing 💕

Very well covered! Great to learn a bit more of the fundamentals of it beyond just going through the motions of using it

The cell orientation doesn't affect the scattering? Or are the cells oriented in somehow? Maybe I missed something.