MTT assay | Cell Viability and Cytotoxicity determination using MTT assay

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Please tell me cells seedling calculation

Great sir.Thank you so much

يا ابن العرث مش فاهمين حاجه

can u please share your slides

Thank u for such good concepts
Appreciate it

Cancer, apoptosis, metabolic activity of cell- MTT assay

Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you

Thanks arpan

Y we call it MTT ?

Please make vedio on Oecd guidelines 407, 408, 409, 410 and on Neutral Red assay

Hi, I have a question regadring with MTT assay😃

Thank u.. clear and very beauty explain

When drug is added into it

On which website I will find this information in written ?

Honestly this was so much clear and helpful ! thank you very much you are amazing !!

This video is really helpful. Thank you for your wonderful presentation, it really helped me in my research study

Arpan, your videos are incredible, please keep going!

Thank you for this video.
I need to have cytotoxicity studies done for several samples of mercury sulphide on healthy cell lines of liver and kidney. Can you help me on where this can be done and what may be the approximate cost? Thanks in advance.

very useful thankyou sir

Amazing explanation sir.thank you so much sir.

I would like to ask if the reduction of tetrazolium takes place in the cytoplasm or in the mitochondria, i.e. the dye also penetrates into the mitochondria

thank you sooo much

Such an awesome video..thank you so much💓💓

We need more of such clips ... very informative and concise

Sir can you make a video on cell invasion and migration assay

Can i use this technique to measure cell viability after autolysis of E. Coli??

Must we wait 2 hours after adding DMSO?

very clear and simple explanation. love it
:)

What are the material and chemicals required for this experiment?

Very well-detailed video!

thank soo much

Nice lecture ,
Sir plzzzzzz explained cell revival,cell subculturing ,cell culture , splitting and passaging etc Also with protocol.

Thank you so much 😭👍🏻

Thank you so much !!!

Can you tell me what is the function of DMSO?

It has really helped me a lot in understanding the test report of my research samples. Please let me know in which stage does the treated sample extract come in contact with the cells

Which app did you use?

Nice video :)
Where are you get the animations (the little pictures)? Is there a website link?

Hello! so does MTT and LDH assays both use yellow tetrazolium salts that get converted into formazan? why is formazan produced different colours? is it bc in mtt the tetrazolium salt contains bromide (MTT) and in LDH - iodine (INT)?

Thank you so much sir for your time and effort :D a very interesting video and it helps me a lot to do my research in my undergraduate study.

That "Hellow!" at the start of your videos is my energy drink these days. Haha!