FlowJo [TITRATION]

Can you present ab titration protocol :
do you start with the manufacturer concentration titer ?and how many dilutions you do and what is the parameter you use (signal/ noise) to chose the optimal ab concentrations.Thank you

Should you not include a live/Dead with your dilutions, as dead cells stain everything?

Please could you provide a video in how to calculate the stain index? This video was fantastic, as I literally did a Ab titration last week.

Awesome video! it really explains an easy way to understand how across titrations the different variations of fluorcrome intensity compared to the NS. Could you post a video using the FlowJo Stainindex plug in? I really want it to work and dont know how it really works. Very best

Very nice video, would be great if you could show us how to calculate the s.i. :)

Hi Aja. Could you make a new video for flowjo to introduce a statistic analysis if you are free?

Thanks,
Fei

Awesome! I'm new in flow cytometry, lots of things to learn. As you said, like the CD11c, we can not see the break in flowjo, have to calculate the SI to find the best concentration to use, could you please tell me how to calculate the SI and make the curve to see which concentration that I can use? Thank you so much.

I just discovered your channel and I am very glad I did. Really appreciate the effort you put in your videos. Thanks a lot! keep going

Very useful video! Thanks! I have a question: When I selected Sample ID, nearly all my events were on the chart edges. I had to change into log axis in order to see the events, but the resolution is bad. Is it because how I acquired my samples? Thanks in advance!

I made a mistake in naming my samples while running flow cytometry. I have already analyzed the data using Flow Jo, and are in wsp format. Is there any way to rename the sample names in analyzed data in Flow jo?

I just discover this série. I really like how clear and concise is the video on one flowjo function. And indicating the other videos on more on the topic

Very helpful

What if I don't have unstained cells .. no negative control

Hello, compliments of the new year to you! Thank you for the video. Is it possible to have the sample ID for each population once concatenated and viewing as - for example - FSC-H on y-axis and Sample ID on x-axis?

Also, if you're familiar with the CBA plugin, are you able to please film a video on how to use the plugin and troubleshoot when there's errors?

Thank you!

All the videos are fantastic! I am dealing with EC50 assessment by flow and in 384 well plate, so I have muliti groups of samples, is there a easy way to get this done without too much repeat - from data input to export. Thank you so much!